Garuda - Garba Rujukan Digital

p-Index From 2019 - 2024

2.374

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Indonesian Journal of Electronics and Instrumentation Systems Jurnal Ilmu Dasar Microbiology Indonesia BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi Global Medical and Health Communication UNEJ e-Proceeding Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI Warta Pengabdian Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi

Kartika Senjarini

Biology Department, Faculty Of Mathematic And Natural Sciences, University Of Jember

Author-ID : 375743

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Dentistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Nursing Physics Public Health Veterinary

Published : 34 Documents Claim Missing Document

Claim Missing Document

Articles

Title

Identification of Immmunogenic Salivary Proteins of Anopheles vagus based on Mass Spectrometry Analysis Dwi Esti Febriyantiningsih; Kartika Senjarini; Rike Oktarianti
Jurnal ILMU DASAR Vol 18 No 2 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Malaria has been prevalent for a long time in tropical developing regions causing great morbidity and mortality. Among the malaria vectors, Anopheles vagus has been known as secondary malaria vector in East Java. Salivary glands of mosquitoes perform various functions for survival of the vectors and also conducive for blood feeding, harbouring of malaria parasites, and eventual parasite transmission. The salivary gland proteomes of An. vagus have not been carried out yet. The aim of our study was to identify and characterize the immunogenic proteins of salivary glands proteins of An. vagus. A proteomic approach combining one-dimensional electrophoresis (1DE) followed by western blot analysis using human sera from healthy people living in an endemic area (Kendal); liquid chromatography mass spectrometry (LC-MS/MS) and bioinformatic analysis was adopted to provide the first direct insight into identification and characterization of salivary proteins of An. vagus. Identification of immunogenic proteins using western blot analysis has revealed three immunogenic bands which had molecular weights of 69, 75 and 232 kDa. Among those proteins analysed by LC-MS/MS, there were alpha,1-4 glucan phosphorylase, putative myosin class I heavy chain which have the highest number of total spectrum count peptide. Other proteins like vitellogenin and heat shock protein 82 (Hsp82) were also identified. The majority of proteins were scrutinized marked for their role in metabolism, cytoskeleton protein and stress response. Keywords: Anopheles vagus, salivary gland, immunogenic, proteomics

Immunogenity of Protein Extract from Salivary Gland of Anopheles aconitus in Malaria Endemic Area Mahful Septiawan; Budayatin Budayatin; Hidayat Teguh Wiyono; Kartika Senjarini
Jurnal ILMU DASAR Vol 18 No 1 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Although malaria had ever been virtually eradicated from Indonesia but currently malaria is recognized as a serious re-emerging threat to public health. This disease is caused by malaria parasite which is transmitted to human host by Anopheles mosquitoes as main vector. It has been widely observed that saliva of mosquito that transmits disease contains several factors that could enhance pathogen infection. Therefore, it should be possible to control pathogen transmission by vaccinating the host against the molecule(s) in saliva that potentiate the infection. However, immunogenic specific component in mosquitoes vectors of Malaria has not yet been identified so far. The objective of this study are to analyze protein profile of SDS-PAGE and to know the immunogity the protein extract of salivary gland from potential vector of Malaria i.e. An. aconitus We used immunogenic reaction between salivary gland extract of these vectors against pool of human sera which were collected from endemic area. The reaction conducted by the dot-blot analyze. SDS-PAGE studies showed 15 major polypeptide bands of 284, 100, 84, 75, 66, 57, 53, 48, 45, 38, 33, 29, 15, 14, and 11 kDa. The dot-blot studies showed that the protein extract of salivary gland from An. aconitus are immunogenic.

Morphological Description of Drosophila melanogaster Wild Type (Diptera:Drosophilidae), Sepia and Plum Strain Husnul Hotimah; Purwatiningsih Purwatiningsih; Kartika Senjarini
Jurnal ILMU DASAR Vol 18 No 1 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Drosophila melanogaster is one of the insects which have a very important role in the development of the genetic science. Drosophila melanogaster have many mutation, recently there are many mutant such as sepia and plum strain. Morgan et al have found 85 strain mutan of Drosophila melanogaster. The purpose of these research was to characterize of morphological from the head, thorax, and abdomen. The result show that the morphological of Drosophilla melanogaster wild type, sepia and plum mutant have many similarities. The difference of them are the eyes color, Drosophila melanogaster wild type has red eye, sepia strain has a dark brown eye and plum has a dark purple eye.

Isolation of Genes Encoding Arthropod Odorant Binding Proteins (OBP), D7 from Salivary Gland Vectors of Malaria: Anopheles sundaicus Nuryatmaja Gora Pawana; Kahar Muzakhar; Kartika Senjarini
Jurnal ILMU DASAR Vol 16 No 1 (2015)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v16i1.621

The isolation of Arthropod Odorant Binding, D7 protein, encoding genes from Anopheles sundaicus and An. maculatus mosquitos as the malaria vectors in Indonesia is necessary to recognize their characteristic. The isolated genes can be used to develop the Transmission Blocking Vaccine (TBV). This research aims to characterize the D7 protein encoding genes from An. sundaicus and An. maculatus through the synthesis of complementary DNA (cDNA) of D7 protein by using D7 protein primer that has been used for the other species of Anopheles. The mosquitos were taken from Dusun Parasputih, Bangsring, Wongsorejo, Banyuwangi, Jawa Timur. Isolation of the salivary gland was done by performing microdisection method and the isolation of the total RNA was done by performing High Pure RNA Isolation Kit (Roche-Germany). Synthesis of cDNA D7 encoding gene and its amplification were performed by using Maxime RT-PCR Premix Kit (iNtRon Biotechnology). The result of the total RNA and RT-PCR were run in agarose gel and visualized under the UV transiluminator. Based on the visualization, we found that the salivary gland total RNA of female An. sundaicus was 500-750 base pair (bp). The RT-PCR visualization showed a band sized below 100 bp and it was concluded not to be the size of the D7 protein encoding gene. An incompatibility of D7 primer from An. gambiae with cDNA template from An. sundaicus was suspected to be the reason of the gene isolation failure.Keywords: gene isolation, D7 protein, salivary gland, Anopheles sundaicus

Characterization Physiology and Molecular Bacteria Symbiont- Entomopathogenic Nematodes based of Gene Sequences Encoding the 16S rRNA District of Bromo Probolinggo Bagus Setiawan; Didik Sulistyanto; Kartika Senjarini
Jurnal ILMU DASAR Vol 18 No 1 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

This study aims to identify entomopathogenic nematodes symbiotic bacteria phenotypically and based on the gene encoding 16S rRNA sequences. Bacterial symbionts of entomopathogenic nematodes, obtained from isolates from the area Wonokerto (WN01) and isolates Sukapura (SP01), Bromo, Probolinggo, two symbiont bacteria was found in entomopathogenic nematodes Steinernema sp. The method used in this study are: the isolation of entomopathogenic nematodes Steinernema sp. and bacterial symbionts conventionally for the identification of phenotypically, after the characterization of bacterial isolates, the isolation of genomic DNA, 16S rRNA PCR, DNA purification and DNA sequence analysis. The results based on phenotypic characterization showed that isolates WN01 and SP01, yellowish white, gram positive, negative bioluminenscene, catalase positive, can not hydrolyze urea, and also can not produce H2S. The results of the gene encoding 16S rRNA sequence can be deduced WN01 isolates have in common with the bacteria Bacillus strain toyonensis BCT 7112, while the SP01 isolates have in common with the bacteria Bacillus strain cereus ATCC 14 579.

The Diversity of Potential Malaria and Dengue Mosquito Vector from Bangsring Village Wongsorejo District Banyuwangi East Java Siti Fat'hiyatul Azkiyah; Kartika Senjarini; Rike Oktarianti; Hidayat Teguh Wiyono; Syubanul Wathon
Jurnal ILMU DASAR Vol 22 No 1 (2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v22i1.13601

Bangsring village in Wongsorejo regency has been reported as malaria endemic area in Banyuwangi since 2011. Understanding the diversity and behavior of mosquito vector in this area will be very impotant in developing vector control program. The masquitoes were collected by landing collection outside and inside house by human bites, we also observed the area around cattle cage. During observation periods, a total 633 masquitos were collected consist of 44% malaria potential vector (Anopheles spp.) 19% Dengue fever (DF) potential vector (Aedes spp.) and 36% were not belong to both of them. Out of total collected Anopheles spp. mosquitoes about 65% were indentified as An. vagus, 25% were An. indevinitus, 8% were An. vagus (limosus), and only 2% as well as 0,04 % were An. supictus and An. kochi. Meanwhile we found 19% of Aedes sp. were Ae. aegypti and 81% were Ae. albopictus. This study showed that the dominan potential malaria’s vector is An. Vagus and the dominan DF vector is Ae. albopictus. Mostly colleted Anopheles sp. were exophagic and zoophilic with the highest activities between 06.00-08.00 pm. This was in contras to Aedes sp. where mosfly found endophilic and it has 2 hightime of activities Aedes sp. between 06.00- 07.00 AM and 05.00-06.00 PM. Keywords: Dengue, Malaria, diversity, Anopheles, Aedes.

Are Bacteria The Main Producers of Hydrolytic Enzymes in Aquatic Environment Kartika Senjarini; Ulf Karsten; Rhena Schumann
Jurnal ILMU DASAR Vol 9 No 1 (2008)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.046 KB)

Bacteria play an important role in the decomposition of organic matter, which is a key process in aquatic microbial food webs as well as its application in water bioremediation processes. DOM (Dissolved Organic Matter) is the major fraction of organic matter in most aquatic environments. Most of DOM is present as high molecular weight compounds that cannot be taken up by bacteria directly. Therefore, they must be hydrolysed enzymatically to be transported across microbial cell membranes. Molecular fluorosensors have been used as artificial substrates to study hydrolytic enzymes in situ. The present study wants to investigate the following hypothesis i.e. bacteria are the main producers of hydrolytic enzyme in aquatic systems. There were not any significant correlations between bacteria and the investigated hydrolases (esterase, peptidase, and β-glucosidase) in the meso- to eutrophic aquatic systems near Rostock city – North East Germany, although a wide range of bacterial abundances were covered. Enzyme saturations were not detected in all samples which may be explained by a high KM indicating a low affinity of enzymes (1) and or the contribution of many enzymes with different kinetics to the respective substrate degradation (2). There is also increasing evidence that bacteria may not be the sole or dominant source of esterases, peptidases and β-glucosidases in aquatic ecosystems. Invertebrate, fungi and other eukaryotes (diatoms, protozoa etc.) must be considered as possible and even important producers of hydrolytic enzymes. Several other factors may influence the correlations of bacteria to hydrolytic enzyme activities, i.e. variations in the species composition (1), a wide ranged variability of hydrolytic activities influenced directly by other substrates (low enzyme affinity), element availability (N and P), temperature and other abiotic factors (2), the existence and persistence of enzymes caused by other (passive) processes (e.g. cell lysis) (3).

Profile of Interleukin-4 and Interferon-γ of Balb/c Mice after Salivary Gland Extract of Anopheles sundaicus s.l. Vaccination and Infected by Plasmodium berghei-ANKA Ali Machrus; Adrial Adrial; Yunita Armiyanti; Hidayat Teguh Wiyono; Kartika Senjarini
Jurnal ILMU DASAR Vol 15 No 2 (2014)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Malaria infection is initiated when sporozoites are inoculated into a vertebrate host via the salivary glands of an Anopheles mosquito. During Anopheles bite, the salivary glands release components that include vasomodulator and immunomodulators. The salivary components of vectors have important role in transmission of pathogen. Therefore, if these components were injected repeatedly into a vertebrate host, it can stimulate host immune system and inhibit the transmission of the pathogen into the host. This could be observed the increasing level of IFN-γ and decreasing level of IL-4 in mice model of malaria after vaccination with salivary gland ekstract (SGE) from An sundaicus s.l. It has also been proven that this mechanisms was related with pathogen of malaria. This was supported by the reduction of parasitemie rate in those mice model after infection by P. Berghei. Keywords: An sundaicus s.l., IFN-γ, IL-4,immunomodulators, salivary gland , TBV

Co-Authors Adrial Adrial Ahmad Tosin Ajeng Maharani Sri Pananjung Ajeng Maharani Sri Pananjung, Ajeng Maharani Ali Machrus Anak Agung Gede Sugianthara Anam, Khairul Ari Satia Nugraha Arif Setiawan Bagus Setiawan Berlian Permata Dewi Erlambang Budayatin Budayatin Cizein Tri Cahyanti Devi Astikaningrum Devi Astikaningrum Dewi Masruroh Didik Sulistyanto DINA FITRIYAH Dita Paramytha Agustin Dwi Esti Febriyantiningsih Esti Utarti Evi Umayah Ulfa Fitria Mutiah Hidayat Teguh Wiyono Husnul Hotimah Ihsanul Azmi Intan Fitri Indrasari Izzay Afkarina Kahar Muzakhar Khasanah, Rochmatul Nuryu Lailly Nur Uswatul Hasanah Loeki Enggar Fitri Mahful Septiawan Mahriani Mahriani Maulana Jauharil Habib Miatin Alvin Septianasari Mochtar Gunawan Wibisono Moh Mirza Nuryady Moh Mirza Nuryady, Moh Mirza Muhammad Khalid Abdullah Mutiah, Fitria Nadya Rismana Fitriani Naura Paramitha Cindy Ardyah Naura Paramitha Cindy Ardyah Nuril Azizah Nuryatmaja Gora Pawana Purwatiningsih Purwatiningsih Ratis Nour Sholichah Renam Putra Arifianto Rhena Schumann Riana Agatha Listiani Rike Oktarianti Sattya Arimurti Silvya Fitri Nur Azizah Siti Fat'hiyatul Azkiyah Sri Mumpuni Wahyu Widajati Sri Mumpuni Wahyu Widajati, Sri Mumpuni Wahyu Sugeng Setyo Utomo Sugeng Setyo Utomo, Sugeng Setyo Sutoyo Sutoyo Syubanul Wathon Syubbanul Wathon, Syubbanul Syubbanul Waton Syubhanul Wathon Teguh Wahju Sardjono Teguh Wahju Sardjono Ulf Karsten Widya Cahyadi Yasir Mubarok Yenny Febriana Ramadhan Abdi Yunita Armiyanti

Title Search

Found 8 Documents Search Journal : Jurnal Ilmu Dasar

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 8 Documents
Search
Journal : Jurnal Ilmu Dasar